Welcome to MemXTerminator Wiki!¶

MemXTerminator is a software for membrane analysis and subtraction in cryo-EM.

MemXTerminator

Overview¶

This software utilizes 2D averages and their corresponding alignment information, employing methods such as Radon transform, cross-correlation, L1 norm, Bezier curves, Monte Carlo simulations, and Genetic Algorithm. It analyzes and subtracts membranes of any shape in cryo-EM, ultimately producing particle stacks and micrographs with membrane signals removed, which are suitable for subsequent membrane protein analysis.

Features¶

Capable of analyzing biological membranes of any shape, including simple lines and arcs, as well as more complex shapes like S or W curves;
Accurately locates and subtracts biological membrane signals;
Utilizes GPU and CUDA acceleration to enhance computational speed;
Features a user-friendly GUI for ease of use.

Requirements¶

This software requires a GPU and CUDA acceleration. So, the installation of CUDA drivers and libraries is necessary.
pyem is also needed to convert cryoSPARC’s .cs files to Relion’s .star format for processing.

Installation¶

For specific installation methods, please refer to the Installation section.

Usage¶

This software has a user-friendly GUI. To use this software, simply type:

MemXTerminator gui &

For detailed usage tutorials, please refer to the Usage section.

License¶

This software is licensed under GPL v3.0.

Acknowledgement¶

Thanks to Jack(Kai) Zhang@Yale MB&B for his guidance.

Contributing¶

Always welcome! This software may still has room for improvement such as updating the usage documentation, improving the GUI design, and enhancing the software's usability.

I am still working on improving this software. More exciting features are on the way!

Contact¶

If you have any questions, please feel free to contact me: zhen.victor.huang@gmail.com